Enzymatic transfer of the acyl group from acyl dihydroyxacetone phosphate to different substrates.
نویسنده
چکیده
The acyl group of acyl dihydroxyacetone phosphate was shown to be enzymatically transferred in guinea pig liver mitochondria to various acceptors such as lysolecithin, lysophosphatidyl ethanolamine and sn-glycerol3-phosphate to form lecithin, phosphatidyl ethanolamine and phosphatidate, respectively. Coenzyme A and Mg*, but not ATP, were required for this reaction. A rapid exchange of acyl group between acyl dihydroxyacetone phosphate and dihydroxyacetone phosphate was also observed.
منابع مشابه
The Acyl-enzyme Intermediate and the Kinetic Mechanism of the Glyceraldehyde 3-phosphate Dehydrogenase Reaction.
The acyl-enzyme compound formed by n-glyceraldehyde 3-phosphate dehydrogenase has been studied by several workers in a variety of ways (l), primarily in experiments at high enzyme concentrations and in many instances with model substrates. In the present investigation an attempt is made to apply kinetic criteria to the mechanism of the reversible oxidative phosphorylation of the natural substra...
متن کاملThe enzymatic exchange of the acyl group of acyl dihydroxyacetone phosphate with free fatty acids.
Acyl dihydroxyacetone phosphate (acyl DHAPl) has been shown to be a precursor of glycerolipids in a variety of systems (1,2). Acyl DHAP has also been found to be the precursor of glycerol ether lipids (1,Z). It has been shown that acyl DHAP reacts with long chain alcohols to form l-O-alkyl dihydroxyacetone-3-phosphate (alkyl DHAPl) (3,4). During the investigation of the formation of the ether b...
متن کاملFunctional role of a distal (3'-phosphate) group of CoA in the recombinant human liver medium-chain acyl-CoA dehydrogenase-catalysed reaction.
The X-ray crystallographic structure of medium-chain acyl-CoA dehydrogenase (MCAD)-octenoyl-CoA complex reveals that the 3'-phosphate group of CoA is confined to the exterior of the protein structure [approx. 15 A (1.5 nm) away from the enzyme active site], and is fully exposed to the outside solvent environment. To ascertain whether such a distal (3'-phosphate) fragment of CoA plays any signif...
متن کاملPurification and kinetic properties of acyl and alkyl dihydroxyacetone phosphate oxidoreductase.
The enzyme NADPH :acyl or NADPH:alkyl dihydroxyacetone phosphate oxidoreductase has been purified from Ehrlich ascites cell microsomes and guinea pig liver mitochondria. The membrane bound enzyme was extracted by the treatment of the particles with a sodium deoxycholate-KClNADPH solution and purified by chromatography on either a Sephadex G-ZOO or a Sepharose 4B column. The enzyme from ascites ...
متن کاملThe subcellular distribution of acyltransferases which catalyze the synthesis of phosphoglycerides.
The distribution of acyltransferase activities in rat liver homogenates is described in relation to that for five other enzymes, each known t o belong to a certain subcellular particle. Five different transfer reactions were studied with 1-acyl-sn-glycero-3-phosphorylcholine, Z-acyl-snglycero-3-phosphorylcholine, l-acyl-sn-glycero-3-phosphorylethanolamine, l-acyl-sn-glycero-3phosphate and sn-gl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Biochemical and biophysical research communications
دوره 57 3 شماره
صفحات -
تاریخ انتشار 1974